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Determination of inhibition type and inhibition constant of coptisine (Cop) on urease. Lineweaver–Burk plots of 1/v versus 1/urea in HPU (A) and JBU (B) in the absence or presence of 1.00, 2.00, or 4.00 mM Cop. (a, c) The inhibition constant Ki was determined from the plot of slope versus Cop concentration. (b, d) The inhibition constant Kis was determined from the plot of intercept versus Cop concentration.
