Table 3.

Identified differences in genes exclusively found between the VIR CH21 and NVIR ch22 strains.

No.	Cl.*	Product	Acronym	CH21 loci	ch22 loci	Protein lengths		Differences
1.	89	Hypothetical protein		BJL64_00820	BJL65_00820	199	199	Seven sense mutations in a highly variable region.
2.	385	Zinc metalloprotease	FtsH	BJL64_02360	BJL65_02355	710	697	One sense mutation. One SNP resulting in a frameshift at the end of the gene. 36 terminal aa changed to 23 different aa.
3.	475	NAD(P)H-dependent oxidoreductase	WrbA	BJL64_02885	BJL65_02880	178	178	One sense mutation.
4.	1221	Choline transporter	BetT	BJL64_06800	BJL65_06835	548	548	One sense mutation.
5.	1368	Hypothetical protein (lipoprotein)		BJL64_07580	BJL65_07610	230	308	A highly variable region shortened by 78 aa in CH21.
6.	1573	Gamma-aminobutyrate permease	GabP	BJL64_08625	[BJL65_09790]	453	— —	Internal stop codon in ch22.
7.	1700	Hypothetical protein		BJL64_09400	BJL65_10565	258	258	One sense mutation.
8.	1766	AAA family ATPase (FtsK operon associated)	VirB4	[BJL64_08695]	BJL65_09860	— —	832	Internal stop codon in CH21.
9.	2127	Malonate transporter	YfdV	BJL64_11910	BJL65_13180	302	302	Two sense mutations.
10.	2185	Conjugal transfer protein (FtsK operon associated)	TpcC	BJL64_00290 BJL64_08710	BJL65_00290 BJL65_09875	353 358	353 358	Four sense mutations. A variable 17 aa region containing 15 sense mutations.
11.	2356	Peptide ABC transporter ATP-binding protein	NikE	[BJL64_13030]	BJL65_14305	— —	249	Internal stop codon in CH21.
12.	2902	Hypothetical protein		BJL64_11025 — — — — — —	BJL65_12290 BJL65_12295	30 — —	30 30	Identical protein sequences. Tandem duplication. A copy with one point mutation.

^*Cluster's number, for detailed protein sequences and alignment see “https://mol058.mol.uj.edu.pl/extra/clusters.htm.”