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. 2018 Sep 10;13:2821–2832. doi: 10.2147/COPD.S172579

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© 2018 Sun et al. This work is published and licensed by Dove Medical Press Limited

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Effects of the isolated nucleosides on NO and iNOS expression in RAW264.7 cells.

Notes: The isolated nucleosides decreased (A) CSE-induced NO production, (B) iNOS mRNA expression, (C) iNOS protein, and (D) its quantitative level in RAW264.7 cells. **P<0.01, CSE-induced group vs control group (without any treatment); ^#P<0.05, ^##P<0.01, nucleoside treatment groups (25 and 50 µg/mL) vs CSE-induced group. All experiments were repeated three times. Data shown as mean ± SEM (n=3).

Abbreviations: CSE, cigarette smoke extract; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; iNOS, inducible nitric oxide synthase; NO, nitric oxide.