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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Virology. 2018 Jul 20;522:193–198. doi: 10.1016/j.virol.2018.07.020

Figure 1. Strategy to delete NEAT1 gene.

Figure 1.

Positions of guide RNAs used to delete the NEAT1 gene are indicated. Positions of PCR primers to verify deletion of NEAT1 gene in genomic DNA are indicated (primer sequences are indicated in Materials and Methods). The genomic sequence of deletions for the J3C9 and J3E5 lines are shown at the bottom of the figure; deletions in both NEAT1 alleles in both cell lines were identical. The sequence reference numbers are from Homo sapiens genomic DNA, chromosome 11:cloneRP11–86708.