Figure 3. Flow cytometry analysis of Jurkat NEAT1 KO cell lines.
A. NEAT1 KO lines J3C9, J3E5, and parental Jurkat cells were culture for 24 hours in the presence of DMSO, PMA/ionomycin, or anti-CD3/CD28 beads. Flow cytometry was used to evaluate CD69 expression and viability of cells (stained with Zombie Violet dye); a representative experiment is shown. B. The bar graphs summarize the data from three independent experiments.