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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Virology. 2018 Jul 20;522:193–198. doi: 10.1016/j.virol.2018.07.020

Figure 6. NEAT1 lncRNAs are down-regulated following T cell activation.

Figure 6.

A. PBMCs from four HIV-infected individuals (obtained from a PBMC bank established by Dr. Roberto Arduino, UT Houston Health Science Center) were cultured with and without PHA for 48 hours, RNA was extracted and NEAT1 lncRNA levels quantified with Real-Time RT-qPCR. PCR primers were in shared sequences between NEAT1_1 and NEAT1_2. B. Resting CD4+ T cells isolated from two healthy blood donors were cultured with and without PHA for 48 hours, RNA was extracted and NEAT1_1 and NEAT1_2 lncRNA levels quantified with Real-Time qPCR. In the left panel, PCR primers were in shared sequences between NEAT1_1 and NEAT1_2; in the right panel, PCR primers were in sequences unique to NEAT1_2.