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(a) ATP-PPi exchange rates of wild-type ubiquitin and CP1, CP2, and CP7 proteins with Ube1. CP1P<0.05 versus wt ub, CP2P<0.05 versus wt ub, and CP7P<0.05 versus wt ub, n=3. (b) Western-blot of E1 transfer of wild-type ubiquitin and CP1, CP2, and CP7 proteins with Ube1.
