Table 2.

Kinetic parameters of ATP-PPi exchange catalyzed by Ube1 and peptides. ^aP<0.05 versus Ube1+P1, ^bP<0.05 versus Ube1+P1, and ^cP<0.05 versus Ube1+P1, n=3.

	Km	kcat	kcat/Km
	(μM)	(min−1)	(μM min−1)
Ube1+ P1	ND	ND	5.24x 10−5
Ube1+C1	651.4±49.3a	2.29±0.59a	3.52 x10−3a
Ube1+C2	611.1±23.1b	3.73±0.87b	6.10 x10−3b
Ube1+C7	542.6±122.1c	5.54±1.37c	1.01 x10−2c
Ube1+ P3	476.1±163.2	6.68±0.77	1.40 x10−2
Ube1+ P4	250.9±55.6	13.98±2.08	5.57x10−2