Table 1.
Primers used for pan-dengue virus (DENV), DENV serotype-specific and Zika virus (ZIKV) RT-PCR. Y = C/T; K = G/T; W = A/T.
| RT-PCR | Sequence | Ref. | |
|---|---|---|---|
| Pan-DENV | Forward | AAGGACTAGAGGTTAKAGGAGACCC | |
| Reverse | CGYTCTGTGCCTGGAWTGATG | [15] | |
| Probe | FAM-TCTGGTCTTTCCAGCGTCAATATGCTGTT-BHQ | ||
| DENV-1 | Forward | GACACCACACCCTTTGGACAA | [17] |
| Reverse | CACCTGGCTGCTACCTCCAT | ||
| DENV-2 | Forward | GACCACACGYAACGGAGAA | [17] |
| Reverse | TCTGTTTTRAACAGRAGACTTTT | ||
| DENV-3 | Forward | GGGAARACCGTCTATCAATA | [17] |
| Reverse | CGCCATAACCAAYTTCATTGG | ||
| DENV-4 | Forward | TGAAGAGATTCTCAACCGGAC | [17] |
| Reverse | AATCCCTGCTGTTGGTGGG | ||
| ZIKV | Forward | CCTTGGATTCTTGAACGAGGA | [18] |
| Reverse | AGAGCTTCATTCTCCAGATCAA |