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. 2018 Jan 29;3(1):14. doi: 10.3390/tropicalmed3010014

Table 1.

Primers and probes used for singleplex and multiplex PCR for A. phagocytophilum and E. chaffeensis.

Target Pathogen Target Gene Sense Primer Anti-Sense Primer Probe
Anaplasma phagocytophilum (singleplex) msp2 GAAGATGAWGCTGATACAGTA CAACHGCCTTAGCAAACT FAM-TTATCAGTCTGTCCAGTAACA-TAMRA
Anaplasma phagocytophilum (multiplex) msp2 GAAGATGAWGCTGATACAGTA CAACHGCCTTAGCAAACT Cy5-TTATCAGTCTGTCCAGTAACA-BHQ1
Ehrlichia chaffeensis vlpt CTAATTCTGATTTACACGAGTCTTC GCATCATCTTCGAATTGAACTTC TAMRA-TTGAGTTACCTGGTCC-BHQ1