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. 2018 Sep 13;13(9):e0203466. doi: 10.1371/journal.pone.0203466

Fig 4. Microdissection of bronchial epithelial cells isolated from WT and ccsPPARγKO lung tissues, and subsequent qRT-PCR analysis for specific mRNAs of peroxisome biogenesis-related and metabolism-related genes, PPARs and mitochondrial PPARα target genes.

Fig 4

(4A-4D). Microdissection procedure of frozen lung sections was depicted. WT and ccsPPARγKO sections were stained with hematoxylin (4A), the bronchiolar epithelium was marked (4B) and with the help of laser energy the marked regions cut out and catapulated (4C) into the lid of an Eppendorf tube (4D). qRT-PCR analysis for the amplification of specific mRNAs of club cell secreting protein 10 (CC10) and ciliated cell marker protein α-tubulin (4E), peroxisomal biogenesis proteins (4F), PTS1-containing enzymes (4G), PTS2-containing enzymes (4H) different isoforms of PPAR nuclear receptors (4I) and PPARα target genes (4J). For gene abbreviations and primer sequences see Table 2. The expression of the house keeping gene HPRT was used for normalization. Values ± SEM represent the mean relative fold induction from three independent experiments. *P ≤0.05; **P ≤0.01; ***P ≤0.001. Arrow heads: microdissected regions. L: lumen, AE: alveolar epithelium. Bars represent: 4B-4D: 50 μm.