Table 2. List of primers used in this study.
Gene target | Gene bank accession no. | Sence primer (5’-3’) | Antisence primer (5’-3’) | PCR product (bp) | Ann. Temp. °C |
---|---|---|---|---|---|
Abcd3 | NM_008991.2 | TCAGAATGGGACGCTCATTGA | TGGCAGCGATGAAGTTGAATAA | 86 | 57.0 |
Acaa1 | AK143187.1 | CAATGAACTGAAGCGTCGTG | CACCACTGTGGCACTCTCTG | 141 | 59.0 |
Acadvl | AK076037.1 | TGGATCAATTTGCCACCGTG | TCAGAGAAGGCACATGACCT | 152 | 58.0 |
Acox1 | NM_015729.2 | CCGCCACCTTCAATCCAGAG | CAAGTTCTCGATTTCTCGACGG | 86 | 61.0 |
Acox2 | NM_001161667.1 | ACGGTCCTGAACGCATTTATG | TTGGCCCCATTTAGCAATCTG | 125 | 57.9 |
Agps | AK_031049 | TGTCCTCCGTGTCTGTTCCT | CATGGTACAACCTGCCCTTC | 141 | 59.4 |
Cat | BC013447 | GGAGAGGAAACGCCTGTGTGA | GTCAGGGTGGACGTCAGTGAAA | 103 | 64.0 |
Cpt1 | AK149688.1 | TCCAAGTATCTGGCAGTCGA | AGTCCATTTTCCTTCCGTGC | 128 | 57.0 |
Cact | AK152436.1 | GTGGCTTTGCAGGGATCTTC | GGTGACTCCTTCTTCTCGGA | 143 | 58.0 |
Cc10 | AK145844.1 | GCCTCCAACCTCTACCATGA | GGACTTGAAGAAATCCTGGGC | 107 | 59.5 |
Gnpat | AK_010896.1 | TGAGGACGTGCAAGCCTTTG | TCCAGAAGCTGACGGGTGAA | 121 | 58.0 |
Mfp1 | NM_023737.3 | AATACAGCGATACCAGAAGCCA | CCAGCTCTAGTCCTCCTCCA | 71 | 59.6 |
Mfp2 | NM_008292.4 | TTAGGAGGGGACTTCAAGGGA | TCGCCTGCTTCAACTGAATCG | 119 | 59.8 |
Pex13 | NM_023651.4 | TGGATATGGAGCCTACGG | CGGTTAAAGCCCAAACCATT | 81 | 57.9 |
Pex14 | NM_019781.2 | GCCACCACATCAACCAACTG | GTCTCCGATTCAAAAGAAGTCCT | 97 | 59.0 |
Pex5 | NM_008995.2 | AATGCAACTCTTGTATCCCGA | GGCGAAAGTTTGACTGTTCAATC | 91 | 58.5 |
Pex7 | NM_008822.2 | GAACACTGTGTGTTGGGTGTG | CTGCTTTTCAGGTTCGGAAG | 138 | 59.5 |
Phyh | AK146753.1 | TGCCAGTTTCCAGCCTGAAC | CCTGGGAGCACAACCAGACA | 125 | 57.0 |
Pparα | AK081709.1 | TCCTTTCTGAATGGGCACTT | TTAACATTGGGCCGGTTAAG | 125 | 55.0 |
Pparβ | AK089913.1 | GCGGGCTCTAGAATTCCATC | CCGTCTTCTTTAGCCACTGC | 137 | 59.4 |
Pparγ | BC021798.1 | TTTTCAAGGGTGCCAGTTTC | CATGGACACCATACTTGAGCA | 128 | 56.0 |
α-tubulin | AK164335.1 | CCTTCTTCAGTGAGACAGGAGC | TCCTTGCCTGTGATGAGCTG | 139 | 57.9 |