Table 1. Sterol flux in N. fowleri.

			Non-inhibited		CYP51	SMT			Sterol Δ8−Δ7−isomerase
##	Metabolitesa	RRTb	NG	NFc	Posaconazolec	Epiminolanosterol	Abafungin	25-Aza	Tamoxifen	AY9944
1	Cholesterol	1	7.6	25.6	26.0	15.6	2.0	12.7	3.0	9.5
	4-Desmethylsterols (w/o cholesterol)		77.6	62.9	23.8	61.9	88.8	74.5	71.9	54.6
2	Cholesta-5,7,22-trienol	1				3.3	4.3	2.9
3	7-Dehydrocholesterol (7DHC)	1.04	10.8	12.5	3.9	9.9	13.8	12.9	4.1	7.5
4	Lathosterol	1.06		0.8			0.2
5	Zymosterol	1.07		0.4		0.1		0.1
6	Cholesta-5,7,24-trienol	1.11				30.8	30.0	38.8
7	Ergosterol	1.11	35.5	26.7	14.2	7.7	20.7	8.8	24.3	22.8
8	Cholesta-7,24-dienol	1.12				1.6	5.5	2.8
9	Cholesta-5,7,22,24-Tetraenol	1.20				6.9	8.6	5.3
10	Ergosta-5,7-dienol	1.21	31.1	21.3	5.7	1.6	5.7	2.9	43.5	24.3
11	Ergost-7-enol	1.22	0.2	1.2
	4-Monomethylsterols		2.1	6.8	48.5	20.8	8.0	10.5	13.9	29.7
12	4α,14α-Dimethylcholest-8-enol	1.08	0.1	1.1	15.5
13	4α-Methylcholest-8-enol	1.11		3.5	0.4	7.6	7.2	2.6	10.4	5.7
14	31-Norlanosterol	1.14	2.0	2.2	29.7	0.5		1.4
15	4α-Methylcholesta-8,24-dienol	1.18				9.1	0.4	1.5	3.5	19.1
16	Δ7-31-norlanosterol	1.24			2.9
17	4α-Methylcholesta-7,24-dienol	1.26				3.6	0.4	5.0		2.9
18	4α-Methylergosta-8,24(28)dienol	1.31								2.0
	4,4-Dimethylsterols		9.6	3.3	1.0	0.9	0.6	0.9	2.3	0.8
19	Lanostanol	1.30	0.5	0.2	0.2	0.4	0.5
20	Cycloartanol	1.32	6.9	1.1
21	Parkeol	1.40	0.3		0.1	0.1		0.3
22	Cycloartenol	1.41	1.9	2.0	0.7	0.4	0.1	0.6	2.3	0.8
	Other sterols		3.1	1.4	0.7	0.8	0.6	1.4	8.9	5.4

^aAll listed sterol structures are shown in Fig 1. The metabolites were quantified based on the total ion current peak areas of each sterol. NF–N. fowleri, NG–N. gruberi

^bRelative retention time compared to cholesterol

^cPreviously published data[22] used here as a comparative companion in the context of the whole pathway.