Fig 4. Effects of different DEN/CCl₄ protocols on AHF analysis (number/area, size and area) and HCA multiplicity of male and female C3H/HeJ mice.

(A, C) male and (B, D) female. Values are mean + S.D or box and whiskers. n = 8 mice/sex/group. DEN 10 or 50 = diethylnitrosamine 10 or 50 mg/kg b.wt. in 0.9% saline at week 2, respectively and CCl₄ = carbon tetrachloride, i.p., 0.25 to 1.50 μL/g b.wt. in 10% corn oil solution for 8 weeks (see Material and methods section). AHF = altered hepatocyte foci; HCA = hepatocellular adenoma. Data were analyzed by Student t or Mann-Whitney test (p<0.05).