Fig 5. Effects of different DEN/CCl₄ protocols on Ki-67 (cell proliferation) and cleaved caspase-3 (apoptosis) immunostaining in surrounding liver tissue of male and female C3H/HeJ mice.

(A, B) male and (C, D) female. (A, C) Representative photomicrographs (40× objective) (scale bar = 20 μm) and (B, D) semiquantitative analysis. Values are mean + S.D. n = 8 mice/sex/group. DEN 10 or 50 = diethylnitrosamine 10 or 50 mg/kg b.wt. in 0.9% saline at week 2, respectively and CCl₄ = carbon tetrachloride, i.p., 0.25 to 1.50 μL/g b.wt. in 10% corn oil solution for 8 weeks (see Material and methods section). Different letters correspond to statistical difference among groups by ANOVA and post hoc Tukey’s test (p<0.05).