Fig 8. Effects of different DEN/CCl₄ protocols on collagen content (Sirius red), α-SMA and CD68 immunostaining in the liver of female C3H/HeJ mice.

(A) Representative photomicrographs (20× objective; scale bar = 50 μm) and (B) semiquantitative analysis. Values are mean + S.D or box and whiskers. n = 8 mice/group. DEN 10 or 50 = diethylnitrosamine 10 or 50 mg/kg b.wt. in 0.9% saline at week 2, respectively and CCl₄ = carbon tetrachloride, i.p., 0.25 to 1.50 μL/g b.wt. in 10% corn oil solution for 8 weeks (see Material and methods section). Different letters correspond to statistical difference by ANOVA or Kruskal Wallis and post hoc Tukey test or Dunn’s method, respectively (p<0.05).