Table 1. Effects of different DEN/CCl4 protocols on body and liver weights, food consumption and serum transaminases levels of male and female C3H/HeJ mice.
| Parameters | Groups1 | ||
|---|---|---|---|
| Saline/Corn oil | DEN 10/CCl4 | DEN 50/CCl4 | |
| Males | |||
| Body weight at week 3 (weaning) (g) | 9.89 ± 2.00a | 9.12 ± 1.01a | 7.42 ± 1.24b |
| Body weight at week 8 (CCl4 onset) (g) | 23.27 ± 2.42a | 22.19 ± 1.65ab | 20.77 ± 1.08b |
| Final body weight (g) | 27.92 ± 2.64a | 27.15 ± 1.21ab | 26.09 ± 1.01b |
| Food consumption (g/mouse/day) | 4.34 ± 0.56a | 4.60 ± 0.57a | 3.76 ± 0.36b |
| Absolute liver weight (g) | 1.39 ± 0.18b | 1.80 ± 0.28a | 1.56 ± 0.17b |
| Relative liver weight (%) | 4.98 ± 0.49b | 6.62 ± 0.97a | 6.00 ± 0.57a |
| ALT (U/L) | 32.90 ± 7.30b | 63.40 ± 10.89a | 62.55 ± 17.75a |
| AST (U/L) | 47.20 ± 7.55b | 67.40 ± 11.92a | 59.46 ± 9.82a |
| Females | |||
| Body weight at week 3 (weaning) (g) | 9.08 ± 2.22a | 7.69 ± 1.05ab | 7.26 ± 1.33b |
| Body weight at week 8 (CCl4 onset) (g) | 18.27 ± 2.31 | 18.06 ± 0.98 | 17.72 ± 1.42 |
| Final body weight (g) | 20.43 ± 2.56 | 21.46 ± 1.34 | 21.67 ± 1.31 |
| Food consumption (g/mouse/day) | 3.71 ± 0.31b | 4.44 ± 0.33a | 3.71 ± 0.26b |
| Absolute liver weight (g) | 0.99 ± 0.13b | 1.28 ± 0.18a | 1.33 ± 0.27a |
| Relative liver weight (%) | 4.88 ± 0.42b | 5.99 ± 0.70a | 6.13 ± 1.00a |
| ALT (U/L) | 27.40 ± 5.58b | 45.57 ± 11.85a | 40.90 ± 4.17a |
| AST (U/L) | 53.55 ± 6.87b | 62.20 ± 9.44ab | 64.60 ± 9.44a |
Values are mean ± S.D. n = 8 mice/sex/group.
1DEN 10 or 50 = diethylnitrosamine 10 or 50 mg/kg b.wt. in 0.9% saline at week 2 and CCl4 = carbon tetrachloride, i.p., 0.25 to 1.50 μL/g b.wt., in 10% corn oil solution for 8 weeks (see Material and methods section). Different letters correspond to statistical difference among groups by ANOVA and post hoc Tukey’s test (p<0.05).