Fig 4. Inhibition of Cdc20 phosphorylation promotes Cyclin B1 and Securin degradation.

A, C, HEK293 and BJAB cells were transfected with Bub1 and different amount of LANA plasmids (5ug, 10ug and 20ug) or 20ug of empty vectors. 48 hours later, cells were collected and western blot was done with indicated antibodies. BC-3 cells were also transfected with Bub1 and used as a reference control for HEK293 and BJAB. B, D, Investigation of the phosphorylation level of H2A and Cdc20 in absence or presence of LANA by knockdown LANA in KSHV positive BC-3 and JSC-1 cells. Cells were collected and western blot was done with indicated antibodies.