Figure 3.

TGFβ induces Foxp3 expression in Tbet⁺CD4⁺ T-cells a Flow cytometry analysis of TβRII expression on Tbet positive and Tbet negative CD4⁺ T cells in the lungs of tumour-bearing WT mice (N = 16). b Experimental design of Flow cytometry analysis of Foxp3 induction in CD4⁺CD62L⁺ T cells, isolated from the spleens of naïve WT mice, cultured under Th1 polarizing conditions for 5 days and subsequently cultured in the presence or absence of TGFβ for another 2 days (N = 3). c,d Flow cytometry analysis of Foxp3 expressing cells gated on Tbet⁺CD4⁺ T cells as well as Tbet⁺Foxp3⁺ (e) Foxp3+Tbet+ cells gated on CD4⁺ T cells in the lungs of tumour-bearing WT and hCD2-ΔkTβRII mice (N = 9–13).