Figure 5.

PD-L1xEGFR induces NK-cell mediated ADCC (A) FaDu cells were mixed with IL-12-pre-treated NK cells at the indicated E:T ratios and in the presence of 5 µg/ml PD-L1xEGFR or control antibodies. (B) FaDu cells were co-cultured with IL-12-pretreated NK cells at an E:T ratio of 2:1 as described in A. (C) LNCaP cells were mixed with PBMCs at an E:T ratio of 5:1 in the presence of 5 µg/ml PD-L1xEGFR or control antibodies. In all experiments apoptosis was determined by flow cytometry using Annexin-V staining procedure. All graphs represent mean ± SD. Statistical analysis in B and C were performed using One-way ANOVA followed by a Bonferroni post-hoc test (*p < 0.05, ** p < 0.01, *** p < 0.001, ns not significant).