Figure 3.

Increased immunogenicity of prime-boost immunization with LADD boost not due to increased antigen load or inflammatory signals from Listeria alone. Six- to ten-week old A2/DR1 mice were immunized with 100 µg pTVG-HP intradermally and then three weeks later with either 1× 10⁶ cfu LADD-PAP (n = 6), 100 µg of pTVG-HP (n = 5), or pTVG-HP administered intradermally with an intraperitoneal injection with 1× 10⁶ cfu LADD-PAP (n = 5) or LADD-empty (n = 5). One week after the last immunization splenocytes were collected for IFNγ ELISPOT analysis as above. Shown are box and whisker plots with the number of IFNγ spot-forming units (SFU) per million splenocytes for each stimulating antigen for all animals per treatment group. Statistical comparisons were made between the first two treatment groups, and third and fourth treatment groups, using a Mann-Whitney U test and no comparisons were found to have p < 0.05.