Figure 4.
Differences in T-cell immunity were not due to antigen presentation by B lymphocytes. Six-to ten-week old A2/DR1 mice were intravenously injected with 250 µg anti-CD20 (clone SA271G2 antibody, panel A), or IgG as a negative control (panel B), nine days before vaccination. Mice were immunized twice three weeks apart with 1× 106 cfu LADD-PAP, 100 µg pTVG-HP, or pTVG-HP followed by LADD-PAP (n = 3 per group). One week after the last immunization splenocytes were collected for IFNγ ELISPOT analysis as above. Shown are box and whisker plots with the number of IFNγ spot-forming units (SFU) per million splenocytes for each stimulating antigen for all animals per group. Statistical comparisons were made using Mann-Whitney U test, and no comparisons between groups treated with IgG versus anti-CD20 were found to have p < 0.05.