Figure 5.
The anti-tumor response elicited by pTVG-HP and LADD-PAP prime/boost was dependent on CD4± T cells elicited. A2/DR1 mice were injected with 250 µg of anti-CD4+ antibody (clone GK1.5) (or IgG as a negative control) one day before tumor implantation (−1), on days three and six after implantation, and weekly afterwards. Tumors were implanted and animals immunized as in Fig. 1. Tumor volumes were measured three times a week. Shown are tumor growth curves (average volume (cm3) +/- SE) for mice immunized weekly with LADD-PAP (panel A), pTVG-HP (panel B), or one immunization with pTVG-HP followed by weekly immunization with 1× 106 cfu LADD-PAP (panel C), in the presence of anti-CD4+ antibody or IgG control (n = 5–6 for each treatment group). Statistical comparisons were made using Mann-Whitney U test at each time point, and comparisons with p<0.05 are represented with an asterisk.