Figure 6.
Induction of PAP-specific MHC class II-restricted immunity was not reduced using higher concentration of DNA for priming immunization. A2/DR1 mice were immunized with 100 µg (n = 5, red) or 1000 µg of pTVG-HP (n = 6, blue) followed three weeks later with 1× 106 cfu LADD-PAP. One week after the last immunization splenocytes were collected for IFNγ ELISPOT analysis as in Fig. 2. Shown are box and whisker plots with the number of IFNγ spot-forming units (SFU) per million splenocytes for each stimulating antigen for all animals per dose treatment group. Statistical comparisons were made using Mann-Whitney U test, and no comparisons between groups treated with the different DNA concentrations were found to have p < 0.05.