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. 2018 Jun 11;7(8):e1461301. doi: 10.1080/2162402X.2018.1461301

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© 2018 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 2. — S100A4 is expressed in different types of cells in the colon. (A-B) Flow cytometry analysis of the phenotypes of S100A4⁺ cells in the colons of S100A4^+/+.GFP mice treated with 3% DSS for 5 days for 2 cycles by staining GFP⁺ cells with CD45, CD11b, F4/80, CD11 c, CD4, CD8 and CD19 antibodies. (C) Double immunohistochemical staining of S100A4 with E-cadherin, CD11b, F4/80, CD4, CD8, B220 and Gr-1 in DSS-induced colon tissues. Nuclei were counter-stained with DAPI. The arrows indicate double-positive cells. Scale bar, 25 μm. (D) S100A4 concentrations in the cultured supernatants of S100A4⁺ CD11b⁺ cells or S100A4⁻ CD11b⁺ cells as detected by ELISA. **P < 0.01.