Figure 6.

NF-κB signaling in Macrophages is critical for the protection against colon tumorigenesis. Groups of S100A4^−/− and WT mice (n = 5 per group) were treated as Fig 5 (A). (A) The protein levels of p-p65, p-β-catenin and p-ERk in colon tissues on day 10 after DSS were analyzed by Western blotting. (B) Sections from DSS-treated colon tissues of S100A4^−/− and WT mice were double stained with F4/80 (green) and p-p65 (red). Scale bar, 50 μm. (C) Sections from DSS-treated colon tissues of S100A4^−/− and WT mice were stained with Ki67, and the percentages of Ki67 in colons are shown. Scale bar, 50 μm. (D-E) RAW cells were cultured without or with S100A4 (200 ng/ml) or RAGE inhibitor for 2 h as indicated. The levels of p65, p-p65, IKBα, p-IKBα, ERK, p-ERK and RAGE were determined by Western blotting.