Figure 7.

Treatment with S100A4 neutralizing antibody attenuates colitis and prevents colitis-associated tumorigenesis. Groups of WT mice (n = 5 per group) were treated with DSS and anti-S100A4 mAb or mIgG 4 times on days 1, 3, 7 and 10. (A) The body weight change and (B) Representative photographs and (C) length of the colon from S100A4^−/− and WT mice on day 10 are shown. *P < 0.05; **P < 0.01. (D) Colon sections were immunostained with F4/80. Scale bar, 25 μm. (E) Numbers of F4/80⁺ cells per HPF (×400) are shown at right. *P < 0.05. (F) Representative photographs of the colons from mice with mIgG or S100A4 neutralizing antibody treatment. (G) Total tumor incidence and (H) incidence of tumors over 4 mm² in AOM-DSS induced CRC model mice with mIgG or S100A4 neutralizing antibody treatment. *P < 0.05; **P < 0.01.