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. 2018 May 24;7(8):e1463946. doi: 10.1080/2162402X.2018.1463946

Figure 1.

Figure 1.

Local and systemic immune responses produced by vaccination at different sites in naïve mice. A. Schema of the experiment. Briefly, female C57BL/6 mice (five to eight weeks old, four/group) were vaccinated with 20 μg/mouse of pNGVL4a-CRT/E7(detox) DNA via IM injection, followed by electroporation in front leg or hind leg twice at seven-day intervals. Peripheral blood lymphocytes were prepared one week after the final vaccination. Peripheral blood lymphocytes, splenocytes, and lymph node (LN) cells were prepared 14 days after the final vaccination. Cells were stained with anti-mouse CD8+ antibody, H2-Db/E7 tetramer, and 7-AAD. B. Representative flow cytometry images of E7-specific CD8+ T cells in peripheral blood one week after the final vaccination. C. Summary bar graph showing E7-specific CD8+ T cells in peripheral blood one week after the final vaccination. D. Summary bar graph showing E7-specific CD8+ T cells in peripheral blood and splenocytes two weeks after the final vaccination. E. Summary bar graph showing both front leg and hind leg vaccination induced E7-specific CD8+ T cells in related draining lymph nodes. Data are presented as mean ± SD. (***p < 0.001, n.s. not significant).