(A) 3D renderings of parasagittal brain sections covering the PBN and STN width, illustrating the different bundles leaving the PBN following a local injection of an anterograde tracer (PHAL). (B) Sagittal sections illustrating a PHAL injection site in the lateral PBN (c), associated with labeled terminals in the medial (a) and lateral (b) STN. PBN labeled terminals contain dense synaptic bouton mainly localized in STN dorsal sector (d–i). Scale bars: a–c = 200 µm, d–i = 20 µm. (C) Schematic illustrating the location of terminals and synaptic boutons in the STN following the injection of biotinylated dextran amine (BDA) in the lateral portion of the rostral PBN (insert box). Abbreviations: IC, inferior colliculus; IS, injection site; PBN, parabrachial nucleus; PPN, pedunculopontin nucleus; SC, superior colliculus; SNc, substantia nigra pars compacta; SNr, substantia nigra pars reticulate; STN, subthalamic nucleus.