Figure 5.

Restoration of migratory activity of MSC NPs in vitro.

Notes: (A) Representative photographs of migrated MSCs through the membrane pores. MSCs were transferred to the upper chamber of the transwell system after exposure to 8 ng/mL Ptx-PLGA NPs for 8 hours. At 1, 3, and 5 days later, migrated MSCs were stained by crystal violet (400×). (B) Percentage of MSC NPs migrating. MSCs were treated with 5, 8, and 10 ng/mL Ptx-PLGA NPs for 8 hours, then cells were washed and seeded onto the transwell system. Numbers of migrating MSCs at 1, 3, and 5 days after seeding were counted. Cells incubated with blank NPs were considered the control *(P<0.05 compared with the number of migrated cells in control group at different time points after drug loading).

Abbreviations: MSC NPs, mesenchymal stem cells loaded with Ptx-PLGA NPs; Ptx, paclitaxel; PLGA, poly(d,l-lactide-co-glycolide); NPs, nanoparticles.