TABLE I.
Procedure of disk-based ELISA by the LASE technique.
| Spin No. | Speed (RPM) |
Time (s) |
Operation |
|---|---|---|---|
| 1 | 0 | 10 | Loading samples and reagents (MB-Ab, Ab-HRP) |
| 2 | 4000 | 10 | Delivering samples and reagents into reaction chambers |
| 3 | 10 | 3600 | Incubation and priming the siphoning channel |
| 4 | 4000 | 100 | Evacuating the liquid in the reaction chamber |
| 5 | 0 | 10 | Loading wash buffer |
| 6 | 4000 | 10 | Aliquoting and delivering the wash buffer into reaction chambers |
| 7 | 10 | 60 | Priming the siphoning channel |
| 8 | 4000 | 10 | Evacuating the liquid in the reaction chamber |
| 9 | 0 | 10 | Loading TMB substrate |
| 10 | 4000 | 10 | Aliquoting and delivering the TMB substrate into the reaction chambers |
| 11 | 10 | 900 | Color development |
| 12 | 10 | 60 | Detection |