Table 1. List of primers used in this study.
Primer category | Primer sequence (5' → 3') | PCR product size (bp) | Use |
---|---|---|---|
ITGA11 | |||
Forward | GTA TTT GTA GGT TTT TTT GGA TTG ATT GT | 178 | Pyrosequencing |
Reverse | ATC CCT ATT TAA AAT ACA CAC AAA AAT TTC | ||
Sequencing | TTG GTT TAT ATA TTT GGT GA | ||
Forward | GGC GCT CAG CCT GTG G | 144 | Real-time PCR |
Reverse | CCC ACG ACC AGC CAC TTA TT | ||
THBS2 | |||
Forward | GGA GGT GGA GAG GAA TTA GTA GGA | 138 | Pyrosequencing |
Reverse | AAA CCT AAC CCC AAT ACT TAT CCT AAC | ||
Sequencing | GTT TTG TAG TAT AAG TTT TAG GG | ||
Forward | CCT YGC AAA TGG GTG TGA CG | 152 | Real-time PCR |
Reverse | AAA GCT GGG GAA GGA AGC TC |
ITGA11, integrin subunit alpha 11; THBS2, thrombospondin 2; PCR, polymerase chain reaction.