Table 2. Functional gene ontology categories enriched in the amnion of the preterm birth group.
| GO Category | GO term | Annotations | % | P-value | Genes |
|---|---|---|---|---|---|
| Biological processes | Hyper methylation and down regulated | ||||
| GO:0007155 | Cell adhesion | 21.053 | 0.039 | ALCAM, NLGN1, ITGA11, THBS2 | |
| GO:0022610 | Biological adhesion | 21.053 | 0.040 | ALCAM, NLGN1, ITGA11, THBS2 | |
| Hypo methylation and up regulated | |||||
| GO:0051276 | Chromosome organization | 13.462 | 0.003 | DOT1L, DNMT3A, EHMT1, HIST1H2BK, HIST2H2BE, GPX4, TOP2A | |
| GO:0006325 | Chromatin organization | 11.538 | 0.005 | DOT1L, DNMT3A, EHMT1, HIST1H2BK, HIST2H2BE, GPX4 | |
| GO:0000302 | Response to reactive oxygen species | 5.769 | 0.022 | SDC1, APOE, GPX4 | |
| GO:0010035 | Response to inorganic substance | 7.692 | 0.024 | SRRT, SDC1, APOE, GPX4 | |
| GO:0048585 | Negative regulation of response to stimulus | 5.769 | 0.037 | PRKCZ, AES, APOE | |
| GO:0006323 | DNA packaging | 5.769 | 0.049 | HIST1H2BK, HIST2H2BE, TOP2A | |
| GO:0030516 | Regulation of axon extension | 3.846 | 0.059 | NRCAM, APOE | |
| GO:0016055 | Wnt receptor signaling pathway | 5.769 | 0.061 | CSNK2A2, AES, TLE2 | |
| GO:0010811 | Positive regulation of cell-substrate adhesion | 3.846 | 0.082 | PRKCZ, RELL2 | |
| GO:0006979 | Response to oxidative stress | 5.769 | 0.088 | SDC1, APOE, GPX4 | |
| Cellular components | Hyper methylation and down regulated | ||||
| GO:0043005 | Neuron projection | 15.789 | 0.053 | ALCAM, MYH14, SAMD4A | |
| GO:0045202 | Synapse | 15.789 | 0.056 | NLGN1, DLG2, SAMD4A | |
| GO:0014069 | Postsynaptic density | 10.526 | 0.075 | NLGN1, DLG2 | |
| Hypo methylation and up regulated | |||||
| GO:0000785 | Chromatin | 5.769 | 0.080 | DNMT3A, HIST1H2BK, HIST2H2BE | |
| GO:0005694 | Chromosome | 7.692 | 0.092 | DNMT3A, HIST1H2BK, HIST2H2BE, TOP2A | |
| Molecular functions | Hyper methylation and down regulated | ||||
| GO:0005509 | Calcium ion binding | 26.316 | 0.023 | SLC8A3, FAM20C, MRC2, ITGA11, THBS2 | |
| Hypo methylation and up regulated | |||||
| GO:0050662 | Coenzyme binding | 5.769 | 0.077 | LDHB, ACBD6, ETFA | |
| GO:0016278 | Lysine N-methyltransferase activity | 3.846 | 0.078 | DOT1L, EHMT1 | |
| GO:0016279 | Protein-lysine N-methyltransferase activity | 3.846 | 0.078 | DOT1L, EHMT1 | |
| GO:0018024 | Histone-lysine N-methyltransferase activity | 3.846 | 0.078 | DOT1L, EHMT1 | |
| GO:0042054 | Histone methyltransferase activity | 3.846 | 0.095 | DOT1L, EHMT1 |
The percentage of gene hypomethylation and up-regulation or hypermethylation and downregulation within each GO category in the amnion in relation to pregnant women are presented. The indicated P-values correspond to modified Fisher exact P-value for gene-enrichment analysis (EASE score). Of 71 examined genes, 52 genes were hypomethylated and up-regulated, and 19 genes were hypermethylated and down-regulated.
GO, gene ontology.