Figure 3.
Blockade of CA9 via APE1/Ref-1 or CA9 inhibition acidifies and kills PDAC cells. (A,B) 10.05 cells were transfected with the indicated siRNAs or treated with the indicated concentrations of APX2009 or APX2014 and exposed to 0.2% O2 for 48 h (N = 3). Images of pH-mediated fluorescence changes are shown in (B). (C–F) 3D Co-cultures with 10.05 (C,E) or Pa03C (D,F) tumor cells (+CAFs) were treated with increasing concentrations of APX3330, APX2009, and APX2014 or SLC-0111 and FC12-531A for 12 days, and fluorescence intensity was measured (N = 3–6 +/−SD, normalized to DMSO ctrl).