Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 13;8:13756. doi: 10.1038/s41598-018-31830-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Alteration of circadian regulatory gene expression by hypophosphatemia in callus and growth plate tissues. (A–J) mRNA expression of central circadian clock genes in callus and growth plate tissue isolated over a 24 hour period 12 days after the mice were placed on a hypophosphatemic diet. Tissues were harvested every three hours from mice in both groups (n = 3, in each group by time points). Error bars represent ±1 standard error of the mean of the 2–3 repeat analyses performed on six samples per group. ^#Indicates peak time point (p < 0.05); A significant diet effect in two-way ANOVA (p < 0.05) is indicated in the upper left of the figure. (K) Analysis of Per2-driven bioluminescence in callus tissues of control (C) and hypophosphatemic (Pi) animals when cultured ex vivo over a 39 hour period in medium containing 1.0 mM or 0.25 mM phosphate, respectively. (L) Bar graphs of detrended data calculating the periodicity of Per2 in control (25.12 ± 1.45 hours) and Pi (27.86 ± 2.45 hours) (p = 0.023). n = 8 in each group.