Table 6.

Antimicrobial activity of Cap18 derivatives against E. coli ATCC25922 LPS mutants and other Enterobacteriaceae.

Cap18 peptide	Antimicrobial activity: MIC μg/ml
	E. coli ATCC25922					S. typhimurium LT2	Y. ruckeri 392/2003
	Wild-type	ΔwaaC	ΔwaaE	ΔwaaF	ΔwaaG
Cap18-original	4–8	2–4	4	2–4	4	4–8	2–4
L17K	≥64	8	8	8	8	16	≥64
I20E	≥64	4	4	4	8	8	32
I24G	≥64	8	8	8	8	8–16	32
L27P	≥64	8	4	4	4	8	32
I24N	≥64	8	16	8	16	32	≥64
I20N	≥64	16	16	8	16	8–16	≥64
I13R	≥64	32	≥64	16	8	16–32	32–64
I24D	≥64	16	16	16	≥64	≥64	≥64
I13P	≥64	32	32	16	32	16–32	≥64
I24D	≥64	16	16	16	≥64	≥64	≥64
I13D	≥64	≥64	≥64	≥64	≥64	≥64	≥64
L17D	≥64	≥64	≥64	≥64	≥64	≥64	≥64
L17P	≥64	≥64	≥64	≥64	≥64	≥64	≥64
I13F	4	2	2	2	2	4	2
I13H	4	2	2	2	2	4	8–16
K16D	4	2	8	2	4	4	4
L6P	8	4	8	4	4	8	64
I13M	8	4	8	4	4	8	4
K16C	8	4	8	4	8	8–16	8–16
K18P	8	2	4	4	4	4	32
G26T	8	4	4	4	4	4–8	2–4

Data are collected as minimal inhibitory concentrations (MICs) according to the Clinical and Laboratory Standards Institute (CLSI) and expressed in μg/ml. All MIC determinations were carried out in in triplicates.