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Time kill curve of C. difficile treated with asiatic acid. C. difficile strains (A) R20291 and (B) 630 were grown in BHI broth medium with various concentrations of asiatic acid. Growth kinetics was followed for 15 h at 1 h interval. 1% DMSO was added to the control sample. Mean values of at least 3 independent measurements (± standard error) are plotted.
