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. 2018 Sep 13;8:13752. doi: 10.1038/s41598-018-30589-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Increased NBEA expression after contextual fear retrieval at the synapse in the dorsal hippocampus was regulated by the mTOR signaling pathway. (A) Schematic representation of the experimental protocol. (B) Injection of rapamycin (49.03 ± 3.28, n = 4) into the dorsal CA1 hippocampus 30 min before contextual retrieval did not alter the freezing level during retrieval compared to injection of DMSO control (37.95 ± 6.934, n = 4). p = 0.1989, t-test, two-tailed. (C) Representative image of NBEA expression with DMSO treatment 30 min before contextual retrieval. (D) Quantification of NBEA expression with DMSO treatment. No retrieval (1.004 ± 0.1315, n = 3) vs 30 min after retrieval (2.038 ± 0.0843, n = 3), *p = 0.0027, t-test, two-tailed. (E) Representative image of NBEA expression with rapamycin treatment 30 min before contextual retrieval. (F) Quantification of NBEA expression with rapamycin treatment. No retrieval (1.005 ± 0.1142, n = 3) vs 30 min after retrieval (0.7149 ± 0.1254, n = 3), p = 0.1625, t-test, two-tailed. (G) Left, Nbea mRNA expression in total lysates (100.2 ± 2.341, n = 9) and the synaptosome fraction (88 ± 3.487, n = 9). Right, Confirmation of the purity of the synaptosome fraction, showing no nuclear contamination using the nuclear marker, histone H1.