Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 13;8:13766. doi: 10.1038/s41598-018-31822-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Acidification of the cell culture medium reduces the global level of histone acetylation. (A) Diagram illustrating a bovine rumen with low pHe and a petri dish containing cell culture medium acidified by addition of HCl. (B) Immunoblots for H3K9ac from fibroblasts cultured in an acidic environment for 4 h. (C) Quantification of H3K9ac levels in fibroblasts from the control group, acidified with HCl or cultured with 1 µM TSA (positive control). The H3K9ac levels were calculated in relation to total histone H3 (H3). Data are presented as fold change in relation to the control group, and values are shown as the mean ± s.e.m. Different letters indicate a significant difference (P < 0.05). The western blot images were cropped for illustrative purposes. The full gels/blots are presented in Supplemental Fig. 5.