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. 2018 Sep 13;8:13766. doi: 10.1038/s41598-018-31822-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cloned zygotes treated with β-hydroxybutyrate (BOHB) present elevated levels of histone acetylation. (A) Diagram illustrating the SCNT procedure and the treatment of early cloned zygotes with BOHB. (B) Immunocytochemical detection of H3K9ac in the nuclei of cloned zygotes treated or not treated with 6 mM of BOHB for 12 h, starting immediately after ionomycin treatment. (C) Quantification of H3K9ac signal intensity of SCNT nuclei. Data as presented as the mean ± s.e.m., and different letters indicate a significant difference with respect to the control group (P = 0.0005). All images were taken at the same magnification (40× and 2× digital zoom) and at the same laser power, thereby enabling direct comparison of signal intensities. The scale bar corresponds to 10 μm. Cont: control zygotes; BOHB: zygotes treated with 6 mM BOHB.