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Flow diagram of experimental design. Five fragments were collected per colony; two were preserved in DMSO, two in LN and one in PFA. PFA-preserved fragment was decalcified before DNA extraction. One fragment per each preservation method was homogenized using either bead beading or crushing method. LN, liquid nitrogen; DMSO, salt saturated dimethyl sulfoxide (DMSO) – EDTA; PFA, paraformaldehyde. Photos by Ed Roberts.
