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. 2018 Sep 7;9:2146. doi: 10.3389/fmicb.2018.02146

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Copyright © 2018 Hernandez-Agreda, Leggat and Ainsworth.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bacterial assemblage composition (top) and structure (bottom) among preservation and homogenization methods in G. edwardsi (left) and I. palifera (right). The taxonomic composition is consistent among methodologies for G. edwardsi (top left); however, the taxonomic structure is distorted when evaluated using relative abundance (bottom left). For I. palifera, the taxonomic composition is not consistent across methodologies, with major discrepancies in classes Gammaproteobacteria, Clostridia, Betaproteobacteria, Bacilli, Alphaproteobacteria, and Actinobacteria (top right). Discrepancies are enhanced when relative abundance is considered (bottom right). For results by colony see Supplementary Figure 5. Major taxonomic classes are presented in the legend, for complete legend see Supplementary Figure 6.