Table 1.
Number of sequences and OTUs per treatment.
| Coral species | Method | N. samples | N. sequences (total) | N. sequences (av. by samples) | N. OTUs (total) | N. OTUs (av. by samples) | |
|---|---|---|---|---|---|---|---|
| Preservation | Homogenization | ||||||
| G. edwardsi | DMSO | Bead beating | 5 | 235,005 | 47,001 | 9,522 | 2,134 |
| DMSO | Crushing | 5 | 110,634 | 22,127 | 6,823 | 1,530 | |
| Liquid nitrogen | Bead beating | 5 | 212,459 | 42,492 | 9,658 | 2,117 | |
| Liquid nitrogen | Crushing | 5 | 211,469 | 42,294 | 10,790 | 2,527 | |
| PFA | Decalcified | 4 | 190,768 | 47,692 | 4,824 | 1,350 | |
| I. palifera | DMSO | Bead beating | 5 | 186,429 | 37,286 | 4,612 | 1,071 |
| DMSO | Crushing | 5 | 140,986 | 28,197 | 4,979 | 1,182 | |
| Liquid nitrogen | Bead beating | 5 | 211,050 | 42,210 | 7,787 | 1,740 | |
| Liquid nitrogen | Crushing | 5 | 146,383 | 29,277 | 3,647 | 890 | |
| PFA | Decalcified | 5 | 476,036 | 95,207 | 7,769 | 1,936 | |
Counts are estimated on raw data after filtering out chloroplast, mitochondria, unidentified, and unassigned OTUs.