FIGURE 4.

Expression of HSP27 in Caco-2 cells. Monolayers of cultured Caco-2 cells were incubated with supernatants of different Lactobacillus and their ppk-derivatives and expression of HSP27 was determined by Western blot in crude extracts. Blots developed with anti-β-actin antibody were used as loading controls and they also served to normalize expression. Protein signals in the blots for each pair of strains come from the same gel. The graph below the blots shows the relative HSP27 expression from three replicates (expression in wild-type strains set at 100%). Asterisks indicate a significant difference between wild type and ppk strains (p < 0.05).