Table 1.

Strains and plasmids used in this study.

Strain/plasmid	Characteristics	Origin or reference
L. casei
BL23	Laboratory strain; wild type	Maze et al., 2010
BL379	BL23 ppk::pRV300; Eryr	Alcantara et al., 2014
L. plantarum
WCFS1	Intestinal isolate	Kleerebezem et al., 2003
BL398	WCFS1 ppk::pRV300; Eryr	This work
Lpp+	Laboratory strain; wild type	This work
Lpp+ pVE6007	Lpp+ carrying pVE6007; repATS, Cmr	This work
Lpp+ pVE6007 pORIppk	Lpp+ carrying pVE6007 and pORIppk; repATS, Cmr, Eryr	This work
Lppk	Lpp+ ppk::pORI19; Eryr	This work
E. coli
DH10B	F− mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara, leu)7697 araD139 galU galK nupG rpsL	Grant et al., 1990
EC101	[F’ traD36 proA+B+ lacIq lacZΔM15] Δ(lac-proAB) supE thi repA+(pWV01). Host for propagating pORI19-derivatives	Law et al., 1995
Plasmid
pRV300	Integrative plasmid; lacZ’, Ampr, Eryr	Leloup et al., 1997
pORI19	Integrative plasmid lacking repA gene; LacZ’, Eryr	Law et al., 1995
pVE6007	Thermosensitive repA from pWV01 (repATS); Cmr	Maguin et al., 1992
pRVppkW	pRV300 with a 504-bp internal fragment of WCFS1 ppk gene cloned at XhoI and EcoRI	This work
pORIppk	pORI19 with a 504-bp internal fragment of Lpp+ ppk gene cloned at PstI and EcoRI	This work

Amp^r, ampicillin resistance; Ery^r, erythromycin resistance; Cm^r, chloramphenicol resistance.