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. 2018 Sep 13;50(9):122. doi: 10.1038/s12276-018-0147-5

Fig. 1. METRNL attenuates insulin resistance in differentiated C2C12 myocytes and soleus skeletal muscle of mice.

Fig. 1

a Western blot analysis of phosphorylation of IRS-1 and Akt and b 2-deoxyglucose uptake in differentiated C2C12 cells treated with palmitate (200 μM) and METRNL (0–200 ng/mL) for 24 h. Human insulin (10 nM) stimulates IRS-1 and Akt phosphorylation for 3 min. c Western blot analysis of IRS-1 and Akt phosphorylation in soleus skeletal muscle of HFD-fed mice treated with METRNL (5 animals/treatment group). Means ± SEM were calculated from three independent experiments. ***P < 0.001 and **P < 0.01 compared to levels in the control or insulin-injected ND-fed groups.!!!P < 0.001,!!P < 0.01, and !P < 0.05 compared to palmitate or insulin-injected HFD-fed groups