Fig. 3. METRNL attenuates inflammation in myotubes.

a Western blot analysis of palmitate-induced NFκB nuclear translocation and IκB phosphorylation in differentiated C2C12 cells treated with 200 μM palmitate and METRNL (0–200 ng/mL) for 24 h. b Western blot analysis of HFD-induced NFκB nuclear translocation and IκB phosphorylation in soleus skeletal muscle of mice treated with METRNL (1 μg/mouse/day) for 8 weeks. Serum analysis of c TNFα and d MCP-1 of mice treated with HFD and METRNL (5 animals/treatment group). Means ± SEM were calculated from five separate animals. ^***P < 0.001 and ^**P < 0.01 compared to control or ND treatment.^!!!P < 0.001,^!!P < 0.01, and ^!P < 0.05 compared to palmitate or HFD treatment