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. 2018 Sep 7;9:2037. doi: 10.3389/fimmu.2018.02037

Figure 2.

Figure 2

Transcriptional regulation of miR-125a~99b~let-7e cluster. (A) Sequence homology between human (chr.19: 52191745-52196592) and murine (chr. 17:17827604-17832451) miR-125a~99b~let-7e loci was analyzed by pairwise sequence local alignment using the TBA alignment program and visualized as a conservation plot generated by the Mulan software. Red blocks identify evolutionary conserved regions (ECR) with >50% identity between the two species over 100 bp segments. The transcription start site (TSS), TATA boxes (black squares), and the putative binding sites for transcription factors are indicated. (B–E) ChIP assays were carried out on human monocytes stimulated or not for 4 h with 25 ng/ml IL-10, 100 ng/ml LPS or 50 ng/ml TGFβ using anti-Pol II (B) Abs or for 12 h with anti-STAT3 (C), anti-SMAD3 (D), or anti-NF-kB (E) antibodies. Q-PCR was carried out using specific primers for the miR-125a~99b~let-7e promoter (black columns in panels B–E) or the miR-155 promoter (white columns in panel B). Results are expressed as fold change over control (mean ± SEM; n = 3). (* < 0.05; ** < 0.01; *** < 0.001).