Figure 3.

Early cII Expression Is Not Affected by DNA Replication

(A) Average cII levels comparing λWT, λP^-, and λcro^-P^- infections. Data from multiple experiments are shown, and solid lines represent the mean of all cells pooled from different experiments. In the first 18 min, λWT and λP^- have similar cII levels. After 24 min of infection, average λWT cII level is higher compared with λP^-. Average cII expression for λcro^-P^- infection is higher throughout the infection.

(B) Distribution of cII mRNA levels at 6, 12, and 18 min after infection. λWT and λP^- have similar cII distributions, whereas λcro^-P^- infection shows higher cII expression levels.

(C) Average DNA level after infection. Fold change is calculated as the ratio of phage DNA to E. coli DNA normalized to time 0 and further normalized to the mean of λP^- and λcro^-P^- data at the corresponding time point. For λWT and λcro^- infection, phage DNA level increases by 256 ± 7-fold and 29 ± 5-fold, respectively, after 40 min of infection. Error bars denote SEM.

See also Figure S5.