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. 2018 Jul 18;6:38–51. doi: 10.1016/j.isci.2018.07.008

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© 2018 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Replication Kinetics and Viral RNA Copy Fluctuations (gRNA and sfRNA) of DENV-1 Lineages and Strains in C6/36 Aedes albopictus Cells

In vitro experiments were conducted at an MOI of 0.1. The y-axis represents virus titers (pfu/mL) whereas the x axis refers to hours post infection (hpi). The bar chart shows the sfRNA:gRNA ratio for each isolate at 24 hpi. SG(EHI)D1/0091Y09, SG(EHI)D1/15834Y11, SG(EHI)D1/18640Y12, and SG(EHI)D1/44259Y12 isolates possess the 21-nucleotide deletion in the hypervariable region of the 3′ UTR (please see also Figure S2). The deletion is absent in SG(EHI)D1/09106Y11; SG(EHI)D1/04009Y13; SG(EHI)D1/30889Y14; SG(EHI)D1/09063Y15; 05K4441DK1/2005; and 05K4443DK1/2005 isolates. D1, DENV-1; GI, genotype I; GIII, genotype III; w/o, without; del, deletion.